cropseq guide puro vector (Addgene inc)
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Addgene inc
cropseq guide puro vector
Cropseq Guide Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc12680425-86-7-9
Average 96 stars, based on 124 article reviews
Cropseq Guide Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc12680425-86-7-9
Average 96 stars, based on 124 article reviews
cropseq guide puro vector - by Bioz Stars,
2026-09
96/100 stars
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Amplification:Article Title: A genome-wide atlas of human cell morphology Article Snippet: In order to prepare pooled plasmid libraries, targeting and non-targeting guide subpools were first individually amplified by dialout PCR using orthogonal primer pairs. . PCR products were purified using the QIAquick PCR Purification Kit (Qiagen LLC #28104). .. The amplified libraries were cloned into the Clone Assay:Article Title: A genome-wide atlas of human cell morphology Article Snippet: In order to prepare pooled plasmid libraries, targeting and non-targeting guide subpools were first individually amplified by dialout PCR using orthogonal primer pairs. . PCR products were purified using the QIAquick PCR Purification Kit (Qiagen LLC #28104). .. The amplified libraries were cloned into the Article Title: High-dimensional phenotyping to define the genetic basis of cellular morphology Article Snippet: .. To functionally validate the rare-variant burden associations, we designed sgRNAs targeting the transcriptional start site (TSS) for each gene using CRISPick software (Doench, 2016, Sanson, 2018). sgRNA oligonucleotides were cloned into the Article Title: High-dimensional phenotyping to define the genetic basis of cellular morphology. Article Snippet: .. CRISPRi sgRNA design, cloning, and virus production To functionally validate the rare-variant burden associations, we designed sgRNAs targeting the transcriptional start site (TSS) for each gene using CRISPick software (Doench, 2016, Sanson, 2018). sgRNA Nature Communications | (2024) 15:347 9 oligonucleotides were cloned into the Software:Article Title: High-dimensional phenotyping to define the genetic basis of cellular morphology Article Snippet: .. To functionally validate the rare-variant burden associations, we designed sgRNAs targeting the transcriptional start site (TSS) for each gene using CRISPick software (Doench, 2016, Sanson, 2018). sgRNA oligonucleotides were cloned into the Article Title: High-dimensional phenotyping to define the genetic basis of cellular morphology. Article Snippet: .. CRISPRi sgRNA design, cloning, and virus production To functionally validate the rare-variant burden associations, we designed sgRNAs targeting the transcriptional start site (TSS) for each gene using CRISPick software (Doench, 2016, Sanson, 2018). sgRNA Nature Communications | (2024) 15:347 9 oligonucleotides were cloned into the Cloning:Article Title: High-dimensional phenotyping to define the genetic basis of cellular morphology Article Snippet: .. To functionally validate the rare-variant burden associations, we designed sgRNAs targeting the transcriptional start site (TSS) for each gene using CRISPick software (Doench, 2016, Sanson, 2018). sgRNA oligonucleotides were cloned into the Article Title: High-dimensional phenotyping to define the genetic basis of cellular morphology. Article Snippet: .. CRISPRi sgRNA design, cloning, and virus production To functionally validate the rare-variant burden associations, we designed sgRNAs targeting the transcriptional start site (TSS) for each gene using CRISPick software (Doench, 2016, Sanson, 2018). sgRNA Nature Communications | (2024) 15:347 9 oligonucleotides were cloned into the Virus:Article Title: High-dimensional phenotyping to define the genetic basis of cellular morphology. Article Snippet: .. CRISPRi sgRNA design, cloning, and virus production To functionally validate the rare-variant burden associations, we designed sgRNAs targeting the transcriptional start site (TSS) for each gene using CRISPick software (Doench, 2016, Sanson, 2018). sgRNA Nature Communications | (2024) 15:347 9 oligonucleotides were cloned into the Construct:Article Title: Tethering distinct molecular profiles of single cells by their lineage histories to investigate sources of cell state heterogeneity Article Snippet: .. Target/sgRNA construct: In order to integrate CRISPR targets and sgRNAs into the genome, we modified the CRISPR:Article Title: Tethering distinct molecular profiles of single cells by their lineage histories to investigate sources of cell state heterogeneity Article Snippet: .. Target/sgRNA construct: In order to integrate CRISPR targets and sgRNAs into the genome, we modified the Modification:Article Title: Tethering distinct molecular profiles of single cells by their lineage histories to investigate sources of cell state heterogeneity Article Snippet: .. Target/sgRNA construct: In order to integrate CRISPR targets and sgRNAs into the genome, we modified the |
